Guides · Ohio · Updated October 23, 2026

Rapid STD Testing in Cincinnati: Walk-In Labs, Hours, and the City Health Department Clinic

Rapid STD testing in Cincinnati, Mason, Hamilton, and Northern Kentucky: walk-in lab addresses and hours, the Cincinnati Health Department walk-in STI testing, and costs.

Rapid STD testing in Cincinnati today

13 private walk-in lab locations in the Cincinnati area. Order online, walk in with no appointment, about 5 minutes, results in 1 to 2 days. Individual tests from $24, 10-test panel $139. Nothing billed to insurance.

Find a Cincinnati Lab → See all 13 locations ↓

Cincinnati’s city health department offers walk-in STI testing on a sliding scale, and private walk-in labs cover the city and the northern suburbs, so rapid STD testing in Cincinnati is available the same day at any budget. This page lists every walk-in lab in Cincinnati, Mason, Fairfield Township, Hamilton, Middletown, Milford, and Fort Mitchell, Kentucky with hours, plus the city clinic. For Columbus and Louisville, see Columbus and Louisville.

The fastest option: order online, walk into a lab today

Private lab draw sites are located along Colerain Avenue and North Bend Road on the west side, on Reading Road, along Montgomery Road toward Kenwood, on Ohio Pike in the east, and on Steger Drive, with more in Mason, Fairfield Township, Hamilton, Middletown, Milford, and Fort Mitchell, Kentucky. Order online, walk in with no appointment, spend about 5 minutes, and get results in 1 to 2 days in a private account. Nothing is billed to insurance and nothing goes on your medical record.

Most sites open at 6:30 or 7 a.m. on weekdays and a few open Saturday mornings. The full list with hours is below.

Cincinnati Health Department, Ambrose H. Clement Health Center

  • Address: 3559 Reading Rd, Suite 101, Cincinnati, OH 45229 (Avondale), per the city’s current page. Older listings show 3101 Burnet Ave.
  • Hours: Monday through Friday, 8 a.m. to 5 p.m.; walk-in STI testing available. A 2025 guide lists walk-in windows of 8:30 to 10 a.m. and 12:30 to 3 p.m.
  • Phone: 513-357-7300
  • Tests: chlamydia, gonorrhea, syphilis, HIV (including rapid), herpes, and hepatitis
  • Cost: sliding fee scale; Medicaid, insurance, and self-pay accepted. Eligibility may be limited to city residents.

Rapid HIV results are available during the visit; lab results take several days. Northern Kentucky residents can use the Northern Kentucky Health Department, and Hamilton County residents outside the city have county options.

Urgent care in Cincinnati

Urgent care centers along Montgomery Road, Colerain Avenue, Beechmont Avenue, and in Mason and Northern Kentucky offer STD testing with an exam. Without insurance, plan on a visit fee of $100 to $200 plus lab charges, with results in 2 to 5 days.

Ohio notes

  • Expedited partner therapy is allowed in Ohio for chlamydia, gonorrhea, and trichomoniasis.
  • Minors can consent to their own STD testing and treatment in Ohio.
  • Students: UC and Xavier health services test students; a private walk-in lab keeps results off university and insurance records.

When to test

Chlamydia and gonorrhea at 2 weeks after exposure, HIV and syphilis at 6 weeks, herpes antibody at 12 weeks. With symptoms, test now and retest after the window.

Which tests to order

The 10-test panel ($139) covers the common infections in one visit. After one known exposure, chlamydia and gonorrhea together ($99) at two weeks and HIV plus syphilis ($49 each) at six weeks is a reasonable minimum.

Testing with a partner in Cincinnati

A lot of people get tested as a couple, at the start of a relationship or before stopping condoms. It works the same way as testing alone, just twice: each of you places your own order, so each of you gets your own private results, then you can walk into the same lab together and be done in about ten minutes.

  • Same tests, same day. Ordering the same panel for both of you keeps the results easy to compare. The 10-test panel is the usual choice; add trichomoniasis if you have vaginal sex.
  • Results are individual. Neither of you can see the other's results unless you share them, which is worth agreeing on before you test.
  • If one of you tests positive, treat both of you at the same time for chlamydia, gonorrhea, or trichomoniasis, even if the other person tested negative, or the infection passes back and forth. Retest in 3 months.
  • A positive result is not proof of recent cheating. Chlamydia, trichomoniasis, herpes, and HPV can go unnoticed for months or years.

Order tests for both of you → Read the full guide to testing as a couple

Frequently asked questions

Where can I get same-day STD testing in Cincinnati?

Order online and walk into any private lab draw site in Cincinnati, Mason, Milford, Hamilton, Middletown, or Northern Kentucky the same day. No appointment, about 5 minutes, results in 1 to 2 days.

Does the Cincinnati Health Department do STD testing?

Yes. The Ambrose H. Clement Health Center, listed by the city at 3559 Reading Rd, Suite 101, offers walk-in STI testing on weekdays between 8 a.m. and 5 p.m., on a sliding fee scale; Medicaid and insurance are accepted. Some listings still show the old Burnet Avenue address, so call 513-357-7300 to confirm.

How much does STD testing cost in Cincinnati without insurance?

Private lab tests are $24 to $59 each or $139 for a 10-test panel, paid directly with no visit fee.

Can I get STD tested on a Saturday in Cincinnati?

A few Cincinnati-area lab locations open Saturday mornings. Order online first and confirm the hours of the location you choose.

Walk-in testing centers in Cincinnati

13 private lab locations in the Cincinnati area accept walk-ins with an online order. No appointment. 5 are open on Saturday. Order first, then walk in; the staff only see a lab order.

Order & pick your location →

Cincinnati 6 locations
Mason 2 locations
Fort Mitchell, KY 1 location
Fairfield Township 1 location
Hamilton 1 location
Middletown 1 location
Milford 1 location

Locations and hours are provided by the testing provider and can change. Confirm the hours shown when you order. This list covers private lab draw sites; public health clinics are listed separately above.

This page is for general information only and is not medical advice. Public clinic details are gathered from official sources and can change; confirm hours before visiting. RapidSTDTests.com is an informational referral website and may be compensated when you order testing through our links.

Other cities: Columbus, OH · Orange County, CA · Newark and North Jersey · Long Island · Hartford · Providence · Boston · Buffalo

Get tested in Cincinnati today

Order in minutes, walk into any of 13 local labs, and get confidential results in 1 to 2 days.

Find a Local Lab →