Guides · Florida · Updated October 2, 2026

Rapid STD Testing in Jacksonville: Walk-In Labs, Hours, and the Duval County Clinic

Rapid STD testing in Jacksonville and Orange Park: walk-in lab addresses and hours, the Duval County health department STD clinic downtown, and what testing costs.

Rapid STD testing in Jacksonville today

40 private walk-in lab locations in the Jacksonville area. Order online, walk in with no appointment, about 5 minutes, results in 1 to 2 days. Individual tests from $24, 10-test panel $139. Nothing billed to insurance.

Find a Jacksonville Lab → See all 40 locations ↓

Jacksonville is spread across the largest city footprint in the country, so rapid STD testing in Jacksonville is mostly about picking the lab on your side of the river. This page lists every walk-in lab in Jacksonville, Orange Park, the Beaches, and Nassau and Clay counties with hours, plus the county health department clinic. For other Florida cities, see Orlando and Miami.

The fastest option: order online, walk into a lab today

Private lab draw sites are concentrated along Beach Boulevard and Atlantic Boulevard on the Southside and toward the Beaches, San Jose Boulevard and Old St. Augustine Road in Mandarin, Riverside near the hospitals, and Dunn Avenue on the Northside, with more in Orange Park, Middleburg, Fernandina Beach, and Yulee. Order online, walk in with no appointment, spend about 5 minutes, and get results in 1 to 2 days in a private account. Nothing is billed to insurance and nothing goes on your medical record.

The earliest sites open at 5 a.m. on weekdays, and about a third open Saturday mornings. The full list with hours is below.

Florida Department of Health in Duval County

  • Address: Central Health Plaza, 515 W. 6th St, Jacksonville, FL 32206 (downtown, north of the Springfield district)
  • Hours: Monday, Wednesday, and Friday 8 a.m. to 5 p.m.; Tuesday and Thursday 8 a.m. to 7 p.m.
  • Walk-in hours for STD services: Monday through Friday 8 to 10 a.m. and 1 to 3 p.m.
  • STD appointments: 904-253-1130. Main line 904-253-1000.
  • Tests: chlamydia, gonorrhea, syphilis, and HIV at low or no cost

Rapid HIV results are available during the visit; lab results take several days. Tuesday and Thursday evenings are the slots that work around a day job, by appointment.

Urgent care in Jacksonville

Urgent care centers are on most major corridors, and most offer STD testing with an exam. Without insurance, plan on a visit fee of $100 to $200 plus lab charges, with results in 2 to 5 days. Best if you have symptoms and want treatment today.

Florida notes

  • Minors can consent to STD testing and treatment in Florida.
  • Expedited partner therapy is permitted in Florida for chlamydia and gonorrhea.
  • Military: Naval Air Station Jacksonville and Mayport personnel can use military medicine, but private testing is the confidential option and has no residency requirement.

When to test

Chlamydia and gonorrhea at 2 weeks after exposure, HIV and syphilis at 6 weeks, herpes antibody at 12 weeks. With symptoms, test now and retest after the window.

Which tests to order

The 10-test panel ($139) covers the common infections in one visit. After one known exposure, chlamydia and gonorrhea together ($99) at two weeks and HIV plus syphilis ($49 each) at six weeks is a reasonable minimum.

Testing with a partner in Jacksonville

A lot of people get tested as a couple, at the start of a relationship or before stopping condoms. It works the same way as testing alone, just twice: each of you places your own order, so each of you gets your own private results, then you can walk into the same lab together and be done in about ten minutes.

  • Same tests, same day. Ordering the same panel for both of you keeps the results easy to compare. The 10-test panel is the usual choice; add trichomoniasis if you have vaginal sex.
  • Results are individual. Neither of you can see the other's results unless you share them, which is worth agreeing on before you test.
  • If one of you tests positive, treat both of you at the same time for chlamydia, gonorrhea, or trichomoniasis, even if the other person tested negative, or the infection passes back and forth. Retest in 3 months.
  • A positive result is not proof of recent cheating. Chlamydia, trichomoniasis, herpes, and HPV can go unnoticed for months or years.

Order tests for both of you → Read the full guide to testing as a couple

Frequently asked questions

Where can I get same-day STD testing in Jacksonville?

Order online and walk into any private lab draw site in Jacksonville, Orange Park, or the Beaches the same day. No appointment, about 5 minutes, results in 1 to 2 days. The Duval County health department also has daily walk-in hours downtown.

Is there free STD testing in Jacksonville?

The Florida Department of Health in Duval County at 515 W. 6th St offers low or no-cost testing for chlamydia, gonorrhea, syphilis, and HIV, with walk-in hours Monday through Friday 8 to 10 a.m. and 1 to 3 p.m. STD appointments: 904-253-1130.

How much does STD testing cost in Jacksonville without insurance?

Private lab tests are $24 to $59 each or $139 for a 10-test panel, paid directly with no visit fee.

Can I get STD tested on a Saturday in Jacksonville?

Yes. About a third of Jacksonville-area lab locations open Saturday mornings. Order online first and confirm the hours of the location you choose.

Walk-in testing centers in Jacksonville

40 private lab locations in the Jacksonville area accept walk-ins with an online order. No appointment. 12 are open on Saturday. Order first, then walk in; the staff only see a lab order.

Order & pick your location →

Jacksonville 24 locations
Orange Park 4 locations
Fernandina Beach 2 locations
Middleburg 2 locations
Saint Augustine 2 locations
St Augustine 2 locations
Yulee 2 locations
Jacksonville Beach 1 location
St Johns 1 location

Locations and hours are provided by the testing provider and can change. Confirm the hours shown when you order. This list covers private lab draw sites; public health clinics are listed separately above.

This page is for general information only and is not medical advice. Public clinic details are gathered from official sources and can change; confirm hours before visiting. RapidSTDTests.com is an informational referral website and may be compensated when you order testing through our links.

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Get tested in Jacksonville today

Order in minutes, walk into any of 40 local labs, and get confidential results in 1 to 2 days.

Find a Local Lab →