Guides · Florida · Updated October 23, 2026

Rapid STD Testing in Naples and Fort Myers: Walk-In Labs, Hours, and the Collier County Clinic

Rapid STD testing in Naples, Fort Myers, Bonita Springs, and Marco Island: walk-in lab addresses and hours, the Collier County health department clinic, and costs.

Rapid STD testing in Naples today

26 private walk-in lab locations in the Naples and Fort Myers area. Order online, walk in with no appointment, about 5 minutes, results in 1 to 2 days. Individual tests from $24, 10-test panel $139. Nothing billed to insurance.

Find a Naples Lab → See all 26 locations ↓

Southwest Florida’s population swells every winter, and rapid STD testing in Naples and Fort Myers is easy to find year-round: about two dozen private walk-in labs, nearly half open Saturday, plus the Collier County health department. This page lists every walk-in lab in Naples, Marco Island, Bonita Springs, and Fort Myers with hours, plus the county clinic. For Tampa Bay and Miami, see Tampa and Miami.

The fastest option: order online, walk into a lab today

Private lab draw sites in Naples are located along Immokalee Road in North Naples, on Pine Ridge Road, near NCH on Neapolitan Way, on Collier Boulevard and Davis Boulevard in East Naples, and on Heritage Trail, with more on Marco Island, in Bonita Springs, and in Fort Myers near HealthPark. Order online, walk in with no appointment, spend about 5 minutes, and get results in 1 to 2 days in a private account. Nothing is billed to insurance and nothing goes on your medical record.

The earliest sites open at 6 a.m. on weekdays, and 12 of 26 open Saturday mornings. The full list with hours is below.

Florida Department of Health in Collier County

  • Address: 3339 Tamiami Trail East, Building H, Suite 145, Naples, FL 34112 (the county government campus in East Naples)
  • Hours: Monday through Friday, 8 a.m. to 5 p.m.
  • Appointments suggested, walk-ins welcome. Phone 239-252-8200.
  • Tests: chlamydia, gonorrhea, syphilis, herpes, rapid HIV, and hepatitis B and C
  • Free screening days: the office runs free walk-in rapid HIV and hepatitis screening days through the year; check the county’s events page for upcoming dates.

Lee County residents (Fort Myers, Cape Coral, Bonita Springs) can use the Florida Department of Health in Lee County. Rapid HIV results are available during public clinic visits; lab results take several days.

Urgent care in Southwest Florida

Urgent care centers along US-41, Airport-Pulling Road, Immokalee Road, and in Fort Myers and Cape Coral offer STD testing with an exam. Without insurance, plan on a visit fee of $100 to $200 plus lab charges, with results in 2 to 5 days.

Florida notes

  • Minors can consent to STD testing and treatment in Florida.
  • Expedited partner therapy is permitted in Florida for chlamydia and gonorrhea.
  • Seasonal residents and visitors: private testing has no residency requirement, and results post to your account wherever you are.

When to test

Chlamydia and gonorrhea at 2 weeks after exposure, HIV and syphilis at 6 weeks, herpes antibody at 12 weeks. With symptoms, test now and retest after the window.

Which tests to order

The 10-test panel ($139) covers the common infections in one visit. After one known exposure, chlamydia and gonorrhea together ($99) at two weeks and HIV plus syphilis ($49 each) at six weeks is a reasonable minimum.

Testing with a partner in Naples

A lot of people get tested as a couple, at the start of a relationship or before stopping condoms. It works the same way as testing alone, just twice: each of you places your own order, so each of you gets your own private results, then you can walk into the same lab together and be done in about ten minutes.

  • Same tests, same day. Ordering the same panel for both of you keeps the results easy to compare. The 10-test panel is the usual choice; add trichomoniasis if you have vaginal sex.
  • Results are individual. Neither of you can see the other's results unless you share them, which is worth agreeing on before you test.
  • If one of you tests positive, treat both of you at the same time for chlamydia, gonorrhea, or trichomoniasis, even if the other person tested negative, or the infection passes back and forth. Retest in 3 months.
  • A positive result is not proof of recent cheating. Chlamydia, trichomoniasis, herpes, and HPV can go unnoticed for months or years.

Order tests for both of you → Read the full guide to testing as a couple

Frequently asked questions

Where can I get same-day STD testing in Naples or Fort Myers?

Order online and walk into any private lab draw site in Naples, Bonita Springs, Fort Myers, or Marco Island the same day. No appointment, about 5 minutes, results in 1 to 2 days.

Does the Collier County Health Department do STD testing?

Yes. The Florida Department of Health in Collier County at 3339 Tamiami Trail East, Building H, Suite 145, tests for chlamydia, gonorrhea, syphilis, herpes, rapid HIV, and hepatitis, weekdays 8 a.m. to 5 p.m. Appointments are suggested and walk-ins welcome. Call 239-252-8200. The office also holds free walk-in rapid HIV and hepatitis screening days.

How much does STD testing cost in Naples without insurance?

Private lab tests are $24 to $59 each or $139 for a 10-test panel, paid directly with no visit fee.

Can I get STD tested on a Saturday in Naples or Fort Myers?

Yes. Nearly half of the area's lab locations open Saturday mornings. Order online first and confirm the hours of the location you choose.

Walk-in testing centers in Naples and Fort Myers

26 private lab locations in the Naples and Fort Myers area accept walk-ins with an online order. No appointment. 12 are open on Saturday. Order first, then walk in; the staff only see a lab order.

Order & pick your location →

Naples 8 locations
Fort Myers 7 locations
Cape Coral 4 locations
Bonita Springs 3 locations
Lehigh Acres 2 locations
Marco Island 2 locations

Locations and hours are provided by the testing provider and can change. Confirm the hours shown when you order. This list covers private lab draw sites; public health clinics are listed separately above.

This page is for general information only and is not medical advice. Public clinic details are gathered from official sources and can change; confirm hours before visiting. RapidSTDTests.com is an informational referral website and may be compensated when you order testing through our links.

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Get tested in Naples today

Order in minutes, walk into any of 26 local labs, and get confidential results in 1 to 2 days.

Find a Local Lab →